Studies in phytosterol biosynthesis. Mechanism of biosynthesis of cycloartenol
نویسندگان
چکیده
منابع مشابه
Two Cycloartenol Synthases for Phytosterol Biosynthesis in Polygala tenuifolia Willd
Oxidosqualene cyclases (OSCs) are enzymes that play a key role in control of the biosynthesis of phytosterols and triterpene saponins. In order to uncover OSC genes from Polygala tenuifolia seedlings induced by methyl jasmonate (MeJA), RNA-sequencing analysis was performed using the Illumina sequencing platform. A total of 148,488,632 high-quality reads from two samples (control and the MeJA tr...
متن کاملMechanism-based enzyme inactivators of phytosterol biosynthesis.
Current progress on the mechanism and substrate recognition by sterol methyl transferase (SMT), the role of mechanism-based inactivators, other inhibitors of SMT action to probe catalysis and phytosterol synthesis is reported. SMT is a membrane-bound enzyme which catalyzes the coupled C-methylation-deprotonation reaction of sterol acceptor molecules generating the 24-alkyl sterol side chains of...
متن کاملConcerning the role of lanosterol and cycloartenol in steroid biosynthesis.
An homogenate of rat liver which was capable of converting 2-3H-lanosta-8,24-dien-3fl-ol to 2-3H-cholest-5-en-3/3-ol failed to convert 2-3H-9, 19-cyclolanost-24-en-30-01 to 2-3Hcholest-5-en-3/3-01. However, both substrates underwent reduction at C-24,25. 2-3H-9, 19-Cyclolanost-24-en-3/?-01 was not converted to 2-3H-lanosta-8,24-dien-3P-ol. Both the presence and biosynthesis (from 2-14C-mevalona...
متن کاملNMR studies of biosynthesis and enzyme mechanism
Recent applications of high-field NMR spectroscopy in biosynthesis will be discussed. The emphasis of the lecture will be on the use of 1H, 3H, '3C and '5N-NMR to focus on individual steps of biochemical processes in the formation of natural products and related enzyme-catalyzed events. In the first set of experiments, pulse labelling and invivo studies are used to uncover biosynthetic sequence...
متن کاملPrinted in Great Britain Studies on Phytosterol Biosynthesis : Metabolism of 24 , 25 - Dihydrolanosterol and Cholesterol in
precursors, including lanosterol, into poriferasterol (III) by Ochromonas malhamensis has been demonstrated (Hall et al., 1969; Lenton et al., 1971). Since a cell-free extract of peas can alkylate lanosterol, cycloartenol or desmosterol but not 24,25-dihydrolanosterol (I) or cholesterol (11), it has been suggested that sterols without a A24-bond cannot be alkylated (Russell et al., 1967). Howev...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1968
ISSN: 0306-3283
DOI: 10.1042/bj1070417